activated charcoal, drug testing and the enterohepatic cycle

 The oral consumption of activated charcoal is not expected to have a significant effect on drug use and drug testing in relation to the enterohepatic cycle. 

 

The oral consumption of activated charcoal

 is believed to work by absorbing toxins and drugs in the following way:


Activated charcoal is a specially processed form of carbon that has a large surface area and a highly porous structure. This unique structure allows activated charcoal to adsorb (not absorb) substances onto its surface through a process called adsorption. Adsorption refers to the adhesion of molecules to the surface of another material without being assimilated or dissolved into it.


When activated charcoal is ingested orally, it travels through the digestive system and comes into contact with various substances present in the gastrointestinal tract. The porous structure of activated charcoal attracts and binds to a wide range of molecules, including toxins and drugs. These molecules adhere to the surface of the charcoal, effectively trapping them within its pores.


The principle behind using activated charcoal to treat poisoning or drug overdoses is that by adsorbing these substances, it may prevent their absorption into the bloodstream. This can reduce their systemic effects and potentially limit their toxicity.


It's important to note that activated charcoal is most effective in adsorbing certain types of drugs and toxins. It works best for adsorbing substances that are relatively large, nonpolar, and not readily absorbed by the body. Examples of such substances include some drugs, chemicals, and certain toxins.


Activated charcoal is not effective against all substances. It may not work well for adsorbing substances such as alcohol, heavy metals, certain acids, or substances that have already been absorbed into the bloodstream. Additionally, it does not reverse the effects of substances that have already exerted their effects on the body.

The enterohepatic cycle

 refers to a complex process that occurs between the liver and the small intestine, involving the absorption, secretion, and re absorption of certain substances. This cycle primarily involves bile acids, which are produced in the liver and play a crucial role in the digestion and absorption of dietary fats.


Here's a step-by-step explanation of the enterohepatic cycle:

Bile acid synthesis: Bile acids are synthesized in the liver from cholesterol. They are then conjugated with the amino acids glycine or taurine to form bile salts.

Bile secretion: Bile salts, along with other components such as cholesterol, phospholipids, and bilirubin, are excreted from the liver as bile. Bile is stored in the gallbladder until it is needed for digestion.

Fat digestion: When dietary fats enter the small intestine, bile salts are released from the gallbladder and secreted into the duodenum (the first part of the small intestine). Bile salts emulsify the fats, breaking them down into smaller droplets, which increases the surface area for enzyme action.

Micelle formation and fat absorption: Bile salts form micelles with the digested fats, aiding in their absorption. The micelles transport the fats to the surface of the intestinal cells (enterocytes), where they are absorbed.

Bile acid reabsorption: After aiding in fat absorption, a portion of the bile salts is actively reabsorbed by the enterocytes in the ileum (the last part of the small intestine) and transported back to the liver through the portal vein.

Bile acid recycling: Once the reabsorbed bile salts reach the liver, they are taken up by hepatocytes (liver cells) and returned to the bile canaliculi (small ducts) within the liver. From there, they are re-secreted into the bile ducts and stored in the gallbladder for future use.


This enterohepatic circulation of bile salts allows for the efficient reuse of bile acids, which would otherwise be excreted in the feces. By recycling bile acids, the enterohepatic cycle helps to conserve energy and maintain the necessary levels of bile salts for effective fat digestion and absorption.

Enterohepatic Recycling

 
Enterohepatic recycling (EHR) is a feedback mechanism resulting from the combined roles of the liver and intestine. EHR begins with drug absorption across the intestine into the portal circulation, followed by uptake into the hepatocytes. Next, drug and or conjugated metabolites are secreted into the bile and returned to the intestine, where drug can be reabsorbed into the circulation, in some cases after deconjugation in the GI tract (Figure 9.10). As described previously, a number of drugs are secreted by the liver into bile, and are therefore capable of undergoing enterohepatic recycling. These include antibiotics, NSAIDS, hormones, opioids, digoxin, and warfarin.

In cases of acute drug intoxications, activated charcoal is a commonly used treatment because of its ability to adsorb materials with a high capacity. Although timely single-dose administration is effective in preventing drug absorption of orally ingested drugs, repeated doses of activated charcoal have been shown to increase drug clearance, resulting in reduced plasma exposure of drug (Figure 9.11). This is the result of decreased enterohepatic recycling and increased drug exsorption from the intestine.

However, when it comes to the enterohepatic cycle and drug use, the impact of oral charcoal consumption is limited. Activated charcoal primarily works in the gastrointestinal tract, where it can bind to drugs present in the digestive system before they are absorbed into the bloodstream. This means that it may be useful in preventing the absorption of drugs that have not yet entered the systemic circulation.


Once a drug has been absorbed into the bloodstream and gone through metabolism in the liver, the enterohepatic cycle is already underway. At this stage, activated charcoal taken orally is unlikely to have a significant impact on the drug's fate in the body. The drug has already been metabolized and has gone through the necessary processes in the liver. Charcoal consumed orally cannot reverse or interrupt this cycle.


Regarding drug testing, the enterohepatic cycle is not directly affected by activated charcoal consumption. Drug tests typically analyze metabolites or traces of drugs in bodily fluids or tissues. These metabolites are the byproducts of drug metabolism in the liver and are not affected by activated charcoal in the digestive system.


In summary, while activated charcoal can be effective in preventing the absorption of drugs in the gastrointestinal tract before they enter the bloodstream, its impact on drugs that have already been absorbed and undergone metabolism is limited. Therefore, oral consumption of charcoal is unlikely to have a significant effect on drug use or drug testing in relation to the enterohepatic cycle.

 

 

 

 

 citing sources -

 David R. Taft, in Pharmacology, 2009

 Timothy A. Bertram, ... Sureshkumar Muthupalani, in Haschek and Rousseaux's Handbook of Toxicologic Pathology (Third Edition), 2013

 Joseph A. Ware, ... Shiew-Mei Huang, in Principles of Clinical Pharmacology (Third Edition), 2012

 https://www.sciencedirect.com/topics/medicine-and-dentistry/enterohepatic-circulation

 https://www.sciencedirect.com/topics/medicine-and-dentistry/enterohepatic-circulation#:~:text=As%20described%20previously%2C%20a%20number,opioids%2C%20digoxin%2C%20and%20warfarin.

 

17. Watson WA. Factors influencing the clinical efficacy of activated charcoal. Drug Intell Clin Pharm. 1987;21:160–166. [PubMed] [Google Scholar]

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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6620762/#:~:text=Activated%20charcoal%20adsorbs%20many%20noxious,8%2C%2015%2C%2016).

 

 

 What is Synthetic Urine ?

The chemical makeup of human urine varies and is influenced by various factors, including an individual's health, physical condition, and diet. Consequently, synthetic urine does not have a standardized formula, and each manufacturer typically uses their own unique substance compositions. In most cases liquid urine is water, urea, creatinine and biocide.  Powdered urine is more technical and contains all the normal organic components such as creatinine and urea, uric acid,  along with various inorganic substances like ammonia, sulphates, chlorides, , phosphates, and others. The pH of the product should range from 5 to 8, while the specific gravity should be between 1.010 and 1.030.

What types of Synthetic Urine are their ?

The synthetic urine products available for purchase typically come in two forms: liquid and powdered. Liquid synthetic urine products are the most common and are somewhat usually ready to use meaning you don't need to drop the powder into the water. All liquid urine products are formulated with biocides such as glutaraldehydes and peroxides, which serve to maintain their stability on store shelves. It's important to note that biocides like glutaraldehydes are screened for in a SVT (Specimien validity test) as well as bromine (spa sanitizer), and peroxides (both are oxidizers and also screened for in validation ) . These liquid urine are typically flat and have no odor and thus can be tossed to request another sample .  

Another liquid urine (Xtream) fail for no odor  

Magnum liquid urine invalid Ph

On the other hand, powdered synthetic urine is designed to be mixed with water prior to use. It contains nothing not found in human urine that can fail a SVT. Some products may also include a chemical heat activator, which is made of chemicals not found in natural human urine that can come up as not human or unacceptable specimen. 

Is synthetic urine detectable?

The detectability of synthetic urine can vary. In the past, it was less likely for a collection center to conduct a SVT to detect adulteration in urine samples they received. It was primarily some thing more legit labs are trained in However, with the widespread availability and marketing of liquid synthetic urine products, collection center ,labs have become more vigilant in validating suspicious samples. There are increasing reports of individuals failing drug tests when using liquid urine products as well as showing positive for meth. Labs may scrutinize samples if they exhibit unusual color and or a lack of odor or show out of range ph among other indicators that raise suspicion being tagged as invalid. 

 Quick fix failed me need help fast

 Then their are reports of how Quick fix comes up hot for THC .

 Why Powdered Synthetic is Better than Liquid Synthetic Urine

In today's fast-paced world, where drug testing has become a common practice, it's crucial to stay informed about the best options available to ensure accurate and reliable results. While both types serve the purpose of mimicking real urine, there are aspects that may lend you to prefer one over the other. Liquids only aspect is that it's pre mixed saving you 5 seconds of time to add the urine, the product still needs to be properly heated. 

 The distinct advantages to using powdered synthetic urine over its liquid counterpart is the absence of biocides. It can be custom formulated with odor and realistic color. The following points will explore the reasons why powdered synthetic urine is the superior choice for passing drug tests with confidence. 

PROPER TEMP  Fake It vs. Clear choice sub solutions

Other Powdered synthetic urine like sub solutions that uses a gimmiky chemical heat activator instead of a quality warming pad.  Not only is this unnecessary it will fail you as testified to by this user.  and as this clear choice user who was detected .

Maintaining Proper temp is easy to do, just carry it properly. 

 

  Accurate Composition 

 Powdered synthetic urine is formulated to match the composition and of real human urine more precisely than liquid synthetic urine. This accuracy is vital because drug tests often measure multiple parameters, including color, pH levels, specific gravity, creatinine levels and in some cases uric acid. By using powdered synthetic urine, you can ensure that all these aspects are perfectly replicated, minimizing the risk of detection during the testing process. 

 The first step in evaluating a urine sample is documentation of the appearance or color, temperature and In some lab they may have a LOP local operation procedure of smelling for any presence of odor.

The absence of these sanitizing agents in powdered synthetic urine allows it to maintain a natural scent, providing a more authentic and realistic urine experience during testing.

Absence of Biocides and Peroxides

The next procedure is the SVT As mentioned a SVT or the Test before the test will ascertain if the sample is human. Analyzing proper specific gravity and creatinine, this liquid urine can do however the rest of the SVT looks for Adulterating agents like biocides ,oxidizers, nitrates, pyridinium chlorochromate (PCC). All Liquid synthetic urine contains these additives to make it shelf-stable, which also renders it odorless. 

  All liquid urine is essentially identical, you can find a dozen different liquid products available but they have obtained licenses for their use by the three three formulating companies. The only difference among them lies in their packaging.

By opting for powdered synthetic urine, you significantly reduce the risk of detection during drug tests and increase the likelihood of obtaining accurate and reliable results.

In conclusion, when it comes to synthetic urine, powdered synthetic urine the absence of detectable additives such as biocides and peroxides make it the superior choice for individuals seeking to pass drug tests with confidence. With powdered synthetic urine, you can have peace of mind knowing that you have a reliable solution at hand whenever the need arises.

 

 In conclusion, when it comes to synthetic urine, powdered synthetic urine outshines its liquid counterpart in several key aspects. Its extended shelf life, accurate composition, customizable dilution, enhanced stability, and ability to produce a natural expected odor make it the superior choice for individuals seeking to pass drug tests with confidence. With powdered synthetic urine, you can have peace of mind knowing that you have a reliable solution at hand whenever the need arises.

 

 

Everything you need to know about THC Self Test

 Opiate /cocaine have higher threshold, THC is lowest thus why lines are dark or light when negative

Both ELISA lab screens and home tests are based on the same fundamental principle of using an Enzyme-Linked Immunosorbent Assay (ELISA) to detect the presence of specific substances, such as drugs or antibodies, in a biological sample.

In both cases, the ELISA technique involves the use of specific antibodies that can bind to the target substance of interest. These antibodies are typically immobilized on a solid surface, such as a test strip or a microplate well. When a sample is introduced, any target substance in the sample can bind to the corresponding antibodies, forming an antigen-antibody complex.


In the context of an at-home drug test, ELISA typically involves the following steps:

Collection of urine sample: The individual being tested provides a urine sample using a provided collection cup or container.

Test kit preparation: The self or at-home drug test kit contains all the necessary components for conducting the ELISA. These kits usually include test strips or panels, reagents, and instructions.

Test strip/panel application: The individual dips the test strip or places the urine sample onto the designated area of the test panel. The test strip or panel typically contains specific antibodies that can bind to the target drugs or their metabolites.

Incubation: After applying the urine sample, the test strip or panel is left to incubate for a specified period, allowing the antibodies to interact with any drugs present in the urine.

Detection: The test strip or panel is then examined to determine the presence or absence of drug substances. In an ELISA-based test, this is often done by observing color changes or using a reader device that measures the intensity of the reaction.

Interpretation of results: The test results are interpreted based on the presence or absence of visible color changes or the numerical values obtained from the reader device. The test kit's instructions will provide guidance on how to interpret the results for each specific drug being tested.

It's important to note that at-home drug tests using the ELISA method can provide an indication of drug use but may not be as accurate as laboratory-based tests. Positive results obtained from an at-home test should ideally be confirmed by a certified laboratory using more advanced and precise techniques, such as gas chromatography-mass spectrometry (GC-MS) or liquid chromatography-mass spectrometry (LC-MS).



ELISA (Enzyme-Linked Immunosorbent Assay)  - SELF DRUG  are commonly used to detect the presence of drugs in biological samples, such as urine or saliva. These tests work by using specific antibodies that can bind to the target drugs, resulting in the appearance of colored lines. The presence or absence of these lines indicates whether a drug is present or not.

In a typical ELISA self drug test, there are two main lines that are observed: the test line and the control line. The test line is designed to capture the target drug, while the control line is used to verify the validity of the test. When interpreting the results, the appearance or absence of these lines is compared.

The test line will only appear if the drug concentration in the sample exceeds that threshold. In this case, a negative result would show a clear control line, indicating that the test worked properly, but no test line is visible because the drug concentration is below the detection threshold. This indicates that the person tested negative for that particular drug.

In an ELISA self-test for THC (the active compound in marijuana), a faint line, even if it is not as strong or complete as the control line, is still considered a negative result based on the predetermined threshold set for the test.

 

ELISA self-tests for THC typically have a specific threshold level for the concentration of THC or its metabolites in the sample. This threshold is the minimum concentration required for the test to produce a positive result. If the concentration of THC or its metabolites in the sample is below this threshold, the test is considered negative.

In some cases, a faint line may appear in the test region where the antibodies are designed to bind with THC or its metabolites. This faint line suggests the presence of a small amount of THC or its metabolites in the sample. However, if the concentration is below the defined threshold, it is still considered negative because it does not exceed the level required for a positive result.

It's important to note that the intensity of the test line can vary depending on factors such as the concentration of the substance in the sample and the sensitivity of the test. However, the interpretation of the test results should be based on the guidelines provided by the specific test kit manufacturer. The instructions will specify the criteria for determining a positive or negative result based on the appearance or absence of the test line.

A self test or at-home urine drug test, specifically using the ELISA (Enzyme-Linked Immunosorbent Assay) method, is a diagnostic tool designed to detect the presence of drugs or their metabolites in a person's urine sample. ELISA is a common laboratory technique used for various types of testing, including drug screening.

FALSE NEGATIVES -Do not over hydrate ,

This seems to be a common issue people thinking extra water helps detox THC ...it does not. what it does is dilute your bladder and if your over hydrated and do a self test it can give a diluted result thus a FALSE NEGATIVE  

Along with over hydrating their are other things that may influence a false result or both positive and negative as you reach a clean point .

An ELISA self-test drug test can give false negatives when plain water is used for a few reasons. ELISA (enzyme-linked immunosorbent assay) is a commonly used technique for detecting the presence of drugs or other substances in biological samples.


Lack of drug presence: ELISA tests work by detecting the presence of specific drug molecules in a sample. If a person has not consumed drugs recently or the drug concentration in their system is very low, it may fall below the detection threshold of the test. In such cases, the test may incorrectly indicate a negative result even though the person may have used drugs.


Dilution effect: Plain water, when used to adulterate or dilute a sample, can reduce the concentration of drugs present in the sample. If someone intentionally adds water to their urine sample to lower the drug concentration, it may fall below the detection limit of the ELISA test. Consequently, the test may produce a false negative result, suggesting the absence of drugs when they were actually present.


Test limitations: ELISA tests have certain limitations and can sometimes yield false negatives even when drugs are present in the sample. Factors such as the sensitivity and specificity of the test, variations in the drug metabolites or compounds being tested, and the potential for cross-reactivity with other substances can all contribute to false negatives.
ELISA LIMITS CREATE NEED FOR VALIDATION SCREEN

A validation screen, also known as a Sample Validity Test (SVT), is used to determine if a sample has been diluted, which can potentially result in a false negative result. Dilution of a sample refers to the act of adding a solvent or liquid to the original sample, thereby reducing the concentration of the target substance present in the sample.

When a sample is diluted, the concentration of the target substance may fall below the detection threshold of the test. As a result, the test may not be able to accurately detect the presence of the substance, leading to a false negative result, indicating the absence of the target substance when it is actually present.

To address this issue, a validation screen or SVT is performed as an additional step in the testing process. The SVT is designed to detect the presence of diluted samples by assessing specific parameters, such as the specific gravity, pH, or the presence of certain adulterants or markers that indicate sample tampering.

The validation screen works by comparing the results of the SVT with predefined cutoff values or ranges. If the SVT indicates that the sample is diluted beyond an acceptable threshold, the test result may be considered invalid or inconclusive. This suggests that the original sample may have been manipulated or tampered with, and further investigation or retesting may be necessary to obtain accurate results.

By including a validation screen in the testing process, laboratories can identify potential sample dilution, which helps ensure the reliability and accuracy of the test results. It serves as a quality control measure to detect and prevent false negative results that may arise due to sample manipulation or dilution.

 

"DETOX " Drinks do not detox THC

"Detox" drinks are often marketed as products that can help remove or detoxify substances like THC (the active compound in marijuana) from the body, particularly from urine samples. However, it's important to understand that these drinks do not actually eliminate or detoxify THC from the system.

When a person consumes a "detox" drink, it typically contains a combination of fluids, vitamins, minerals, and herbal extracts. These ingredients may have diuretic properties, meaning they increase urine production and frequency. As a result, the urine becomes more diluted.

The idea behind using a "detox" drink is that by diluting the urine, the concentration of THC or its metabolites may temporarily fall below the threshold level that is typically used in drug tests. This dilution can lead to a short-term window where the drug test may yield a negative result.

However, it's important to note that the effects of these drinks are temporary. The body will continue to metabolize THC over time, and the levels in urine will return to their previous concentration. Once the effects of the "detox" drink wear off, the concentration of THC metabolites in the urine will gradually rise again, potentially surpassing the threshold for detection in drug tests.

It is also worth mentioning that many drug testing laboratories are aware of the practice of using "detox" drinks and have implemented measures to detect dilution. They may use additional tests, such as checking the specific gravity or creatinine levels, to assess the integrity of the urine sample. If a sample is found to be excessively diluted, it may be considered invalid, leading to further testing or potential consequences.

In summary, "detox" drinks do not actually remove THC from the body or detoxify it. They temporarily dilute urine, which may provide a short-term window of negative test results. However, these effects are temporary, and the body will continue to metabolize THC naturally over time, leading to the return of THC metabolite levels to normal.

 

 

FAKE IT Synthetic Urine-The perfect solution when natural detoxification is not an option. 

FAKE IT Synthetic Urine offers a reliable and effective alternative for individuals who need to pass urine drug tests with confidence. Unlike risky dilution methods, which may lead to negative dilute results and raise suspicion, FAKE IT Synthetic Urine provides a consistent and undetectable substitute. With its carefully formulated composition, including key components found in real urine, FAKE IT Synthetic Urine offers a realistic solution that mimics the characteristics and properties of natural urine. Say goodbye to the uncertainties of dilution and embrace the peace of mind that comes with FAKE IT Synthetic Urine - the preferred choice for those seeking a reliable substitute when natural detoxification is not feasible.