Everything you need to know about THC Self Test

 Opiate /cocaine have higher threshold, THC is lowest thus why lines are dark or light when negative

Both ELISA lab screens and home tests are based on the same fundamental principle of using an Enzyme-Linked Immunosorbent Assay (ELISA) to detect the presence of specific substances, such as drugs or antibodies, in a biological sample.

In both cases, the ELISA technique involves the use of specific antibodies that can bind to the target substance of interest. These antibodies are typically immobilized on a solid surface, such as a test strip or a microplate well. When a sample is introduced, any target substance in the sample can bind to the corresponding antibodies, forming an antigen-antibody complex.


In the context of an at-home drug test, ELISA typically involves the following steps:

Collection of urine sample: The individual being tested provides a urine sample using a provided collection cup or container.

Test kit preparation: The self or at-home drug test kit contains all the necessary components for conducting the ELISA. These kits usually include test strips or panels, reagents, and instructions.

Test strip/panel application: The individual dips the test strip or places the urine sample onto the designated area of the test panel. The test strip or panel typically contains specific antibodies that can bind to the target drugs or their metabolites.

Incubation: After applying the urine sample, the test strip or panel is left to incubate for a specified period, allowing the antibodies to interact with any drugs present in the urine.

Detection: The test strip or panel is then examined to determine the presence or absence of drug substances. In an ELISA-based test, this is often done by observing color changes or using a reader device that measures the intensity of the reaction.

Interpretation of results: The test results are interpreted based on the presence or absence of visible color changes or the numerical values obtained from the reader device. The test kit's instructions will provide guidance on how to interpret the results for each specific drug being tested.

It's important to note that at-home drug tests using the ELISA method can provide an indication of drug use but may not be as accurate as laboratory-based tests. Positive results obtained from an at-home test should ideally be confirmed by a certified laboratory using more advanced and precise techniques, such as gas chromatography-mass spectrometry (GC-MS) or liquid chromatography-mass spectrometry (LC-MS).



ELISA (Enzyme-Linked Immunosorbent Assay)  - SELF DRUG  are commonly used to detect the presence of drugs in biological samples, such as urine or saliva. These tests work by using specific antibodies that can bind to the target drugs, resulting in the appearance of colored lines. The presence or absence of these lines indicates whether a drug is present or not.

In a typical ELISA self drug test, there are two main lines that are observed: the test line and the control line. The test line is designed to capture the target drug, while the control line is used to verify the validity of the test. When interpreting the results, the appearance or absence of these lines is compared.

The test line will only appear if the drug concentration in the sample exceeds that threshold. In this case, a negative result would show a clear control line, indicating that the test worked properly, but no test line is visible because the drug concentration is below the detection threshold. This indicates that the person tested negative for that particular drug.

In an ELISA self-test for THC (the active compound in marijuana), a faint line, even if it is not as strong or complete as the control line, is still considered a negative result based on the predetermined threshold set for the test.

 

ELISA self-tests for THC typically have a specific threshold level for the concentration of THC or its metabolites in the sample. This threshold is the minimum concentration required for the test to produce a positive result. If the concentration of THC or its metabolites in the sample is below this threshold, the test is considered negative.

In some cases, a faint line may appear in the test region where the antibodies are designed to bind with THC or its metabolites. This faint line suggests the presence of a small amount of THC or its metabolites in the sample. However, if the concentration is below the defined threshold, it is still considered negative because it does not exceed the level required for a positive result.

It's important to note that the intensity of the test line can vary depending on factors such as the concentration of the substance in the sample and the sensitivity of the test. However, the interpretation of the test results should be based on the guidelines provided by the specific test kit manufacturer. The instructions will specify the criteria for determining a positive or negative result based on the appearance or absence of the test line.

A self test or at-home urine drug test, specifically using the ELISA (Enzyme-Linked Immunosorbent Assay) method, is a diagnostic tool designed to detect the presence of drugs or their metabolites in a person's urine sample. ELISA is a common laboratory technique used for various types of testing, including drug screening.

FALSE NEGATIVES -Do not over hydrate ,

This seems to be a common issue people thinking extra water helps detox THC ...it does not. what it does is dilute your bladder and if your over hydrated and do a self test it can give a diluted result thus a FALSE NEGATIVE  

Along with over hydrating their are other things that may influence a false result or both positive and negative as you reach a clean point .

An ELISA self-test drug test can give false negatives when plain water is used for a few reasons. ELISA (enzyme-linked immunosorbent assay) is a commonly used technique for detecting the presence of drugs or other substances in biological samples.


Lack of drug presence: ELISA tests work by detecting the presence of specific drug molecules in a sample. If a person has not consumed drugs recently or the drug concentration in their system is very low, it may fall below the detection threshold of the test. In such cases, the test may incorrectly indicate a negative result even though the person may have used drugs.


Dilution effect: Plain water, when used to adulterate or dilute a sample, can reduce the concentration of drugs present in the sample. If someone intentionally adds water to their urine sample to lower the drug concentration, it may fall below the detection limit of the ELISA test. Consequently, the test may produce a false negative result, suggesting the absence of drugs when they were actually present.


Test limitations: ELISA tests have certain limitations and can sometimes yield false negatives even when drugs are present in the sample. Factors such as the sensitivity and specificity of the test, variations in the drug metabolites or compounds being tested, and the potential for cross-reactivity with other substances can all contribute to false negatives.
ELISA LIMITS CREATE NEED FOR VALIDATION SCREEN

A validation screen, also known as a Sample Validity Test (SVT), is used to determine if a sample has been diluted, which can potentially result in a false negative result. Dilution of a sample refers to the act of adding a solvent or liquid to the original sample, thereby reducing the concentration of the target substance present in the sample.

When a sample is diluted, the concentration of the target substance may fall below the detection threshold of the test. As a result, the test may not be able to accurately detect the presence of the substance, leading to a false negative result, indicating the absence of the target substance when it is actually present.

To address this issue, a validation screen or SVT is performed as an additional step in the testing process. The SVT is designed to detect the presence of diluted samples by assessing specific parameters, such as the specific gravity, pH, or the presence of certain adulterants or markers that indicate sample tampering.

The validation screen works by comparing the results of the SVT with predefined cutoff values or ranges. If the SVT indicates that the sample is diluted beyond an acceptable threshold, the test result may be considered invalid or inconclusive. This suggests that the original sample may have been manipulated or tampered with, and further investigation or retesting may be necessary to obtain accurate results.

By including a validation screen in the testing process, laboratories can identify potential sample dilution, which helps ensure the reliability and accuracy of the test results. It serves as a quality control measure to detect and prevent false negative results that may arise due to sample manipulation or dilution.

 

"DETOX " Drinks do not detox THC

"Detox" drinks are often marketed as products that can help remove or detoxify substances like THC (the active compound in marijuana) from the body, particularly from urine samples. However, it's important to understand that these drinks do not actually eliminate or detoxify THC from the system.

When a person consumes a "detox" drink, it typically contains a combination of fluids, vitamins, minerals, and herbal extracts. These ingredients may have diuretic properties, meaning they increase urine production and frequency. As a result, the urine becomes more diluted.

The idea behind using a "detox" drink is that by diluting the urine, the concentration of THC or its metabolites may temporarily fall below the threshold level that is typically used in drug tests. This dilution can lead to a short-term window where the drug test may yield a negative result.

However, it's important to note that the effects of these drinks are temporary. The body will continue to metabolize THC over time, and the levels in urine will return to their previous concentration. Once the effects of the "detox" drink wear off, the concentration of THC metabolites in the urine will gradually rise again, potentially surpassing the threshold for detection in drug tests.

It is also worth mentioning that many drug testing laboratories are aware of the practice of using "detox" drinks and have implemented measures to detect dilution. They may use additional tests, such as checking the specific gravity or creatinine levels, to assess the integrity of the urine sample. If a sample is found to be excessively diluted, it may be considered invalid, leading to further testing or potential consequences.

In summary, "detox" drinks do not actually remove THC from the body or detoxify it. They temporarily dilute urine, which may provide a short-term window of negative test results. However, these effects are temporary, and the body will continue to metabolize THC naturally over time, leading to the return of THC metabolite levels to normal.

 

 

FAKE IT Synthetic Urine-The perfect solution when natural detoxification is not an option. 

FAKE IT Synthetic Urine offers a reliable and effective alternative for individuals who need to pass urine drug tests with confidence. Unlike risky dilution methods, which may lead to negative dilute results and raise suspicion, FAKE IT Synthetic Urine provides a consistent and undetectable substitute. With its carefully formulated composition, including key components found in real urine, FAKE IT Synthetic Urine offers a realistic solution that mimics the characteristics and properties of natural urine. Say goodbye to the uncertainties of dilution and embrace the peace of mind that comes with FAKE IT Synthetic Urine - the preferred choice for those seeking a reliable substitute when natural detoxification is not feasible.

Creatine used in Dilution for drug testing how its being done wrong.

Creatine used in Dilution  for drug testing how its being used wrong.  

 Dilution is ...

Dilution refers to the process of decreasing the concentration of a urine sample by consuming excessive fluids with the intention of manipulating a negative outcome on a drug test. Another approach is consuming substances like diuretics (e.g., coffee, cranberry juice) that increase urine output.However, it's important to note that urine drug tests have measures in place to detect dilution attempts. 

Laboratories typically analyze multiple parameters in the urine, including creatinine levels, specific gravity, and pH, to assess the sample's validity. Diluted urine often has low creatinine levels and a lower specific gravity compared to normal urine, indicating that it has been tampered with.

laboratories routinely analyze samples for creatinine and specific gravity levels. Creatinine is a waste product generated by muscles and serves as a measure of urine concentration. Specific gravity, on the other hand, measures the ratio of water to other substances in urine and is another indicator of concentration. Typical creatinine levels in urine range between 20 and 400 mg/dL, while normal specific gravity falls between 1.002 and 1.030. Specimens with a creatinine level above 2 mg/dL or below 20 g/dL, and a specific gravity lower than 1.001 or 1.003, are considered diluted.

If a test is determined to be a negative-dilute result, it is highly likely that an employer will request a second test regardless of the underlying reason. However, in some cases, employers may accept a doctor's note stating that an individual has a shy bladder, which requires consuming large amounts of water to produce a urine specimen, as a valid explanation for a dilute test.

The outcomes of a positive dilute test are considered equivalent to any other positive test. The presence of drugs is detected, and the consequences remain the same.

 In drug testing scenarios, dilution is generally considered an adulteration method and is not a reliable way to pass a drug test. Laboratories are aware of these techniques and have established protocols to detect diluted samples. A negative dilute result occurs when a urine specimen tests Negative  for a particular drug metabolite while also having a crashed specific gravity and creatinine level.

 If a diluted sample is identified, it may be deemed invalid, and further testing or consequences may be implemented, such as retesting or considering it a positive result.

 

Methods of Dilution

 Various items are inevitably added to a standard dilution of consuming a liter a hour of some sports drink and diuretics. most notably would be creatine, certo or pectin , and some times you hear people saying to use charcoal. People cant help but think they are improving things regardless if they have any proof to the claim .

adding random things are pointless for one , your still diluting with tons of fluids.

If items like pectin were so important then why don't they drink it with a cup of water instead of a gallon ?  well besides that fact it would turn to a glob in their stomach .  Pectin is just fruit fiber and it may help you create a bowel movement its not releasing THC from body fat. Same goes for charcoal, which is consumed by mouth into the stomach where it is usually used to aid in poisoning JUST CONSUMED AND STILL IN THE STOMACH.  


consuming creatine will not give you extra creatinine levels

How Creatine Works

 Creatine is a substance found naturally in muscle cells. It helps your muscles produce energy during heavy lifting or high intensity exercise.

 Taking creatine as a supplement is very popular among athletes and bodybuilders. They use it to gain muscle, enhance strength, and improve exercise performance

About half of your body’s creatine stores come from the food you eat — especially red meat and seafood — and the rest is made in your liver and kidneys from amino acids. About 95% of the body’s creatine is stored in the muscles, mainly in the form of phosphocreatine. The other 5% is found in the brain and testes .

Creatine monohydrate absorption is close to 100% [27], and when 2 g of Cr is consumed in an aqueous solution, it reaches peak plasma concentration in approximately 1 h. 

 
During exertion, your muscles convert the stored creatine molecules into energy, which assists in muscle contraction. After being used for energy, creatine is broken down into creatinine, which re-enters the bloodstream en route to the kidneys. 

 
 When you supplement, you increase your stores of phosphocreatine. This is a form of stored energy in the cells. It helps your body produce more of a high energy molecule called ATP.

Most of the creatine in the body is stored as phosphocreatine (66%), while the remaining portion exists as free creatine (33%). On average, the total creatine pool (phosphocreatine + free creatine) in the muscles of a normal-sized person is around 120 grams. Each day, about 1 to 2% (2-3 grams) of the total creatine pool is broken down and converted into creatinine within the muscles. The creatinine is then excreted in the urine.

The body has the capacity to store up to 160 grams of creatine. Therefore, one can think of the normal creatine content in the muscles (approximately 120 grams) as a gas tank that is about 75% full. Scientific studies consistently report that dietary supplementation of creatine can increase muscle creatine and phosphocreatine content by 10-40%. Consequently, supplementing with creatine allows individuals to fill up their creatine storage tank to about 150-160 grams (25-30%). However, people may respond differently to creatine supplementation, with some experiencing increases in muscle creatine content as little as 5-15%, while others may see an increase of 15-40%.

TYPES OF CREATINE 

Creatine monohydrate and creatine ester are two forms of creatine, a popular supplement known for its potential to enhance athletic performance. Let's delve into the differences in absorption and their potential impact on creatinine levels: 

1. Creatine Monohydrate: 

• Absorption: Creatine monohydrate is well-researched and has been shown to be efficiently absorbed by the body. It is the most studied and commonly used form of creatine. 

• Creatinine Levels: Creatinine is a byproduct of creatine metabolism. While creatine monohydrate supplementation may lead to a slight increase in creatinine levels, this is generally considered normal and not indicative of kidney dysfunction. It's crucial to stay hydrated when using creatine to support kidney function. 

2. Creatine Ester: 

• Absorption: Creatine ester is a form of creatine that has been chemically altered to potentially improve absorption. However, research on creatine ester is limited compared to creatine monohydrate, and some studies suggest that the ester form may not be as effective in increasing muscle creatine levels. 

 it has been found that under normal physiological conditions, creatine ethyl ester is very unstable and is largely converted into creatinine in the gastrointestinal tract, where it is also absorbed. Spillane et al¹³ demonstrate this in an experiment in which serum creatine and creatinine measurements were taken from healthy volunteers who either consumed creatine ethyl ester, creatine monohydrate or a placebo. It was found that serum creatinine levels approximately tripled after the ingestion of creatine ethyl ester, while serum creatine only marginally increased. The ingestion of creatine monohydrate caused serum creatine to increase significantly and only caused a marginal increase in serum creatinine.

Therefore, regardless of the small amount consumed during a dilution method, it does not convert into creatinine but rather gets stored. Additionally, the amount of creatinine in so-called detox drinks like Q Carbo is extremely insignificant. Your body only excretes what it breaks down, which means you need to regularly consume creatine and engage in heavy weight lifting to create the waste material that is creatinine.

 HOWEVER  HERE IS THE GOOD NEWS 

There IS a way you can get  creatinine  not creatine in volume you need during dilution. You will need to prepare it ahead of time. 20 grams of creatinine mono hydrate in orange juice, (this is needed for the acid ) and store at room temp for 3 days. Now you have creatinine that can be rejected and pissed out .  

The effect of creatine ingestion on urinary creatinine concentration: Does supplementation mask a heavy dilution?

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4170516/

https://pubmed.ncbi.nlm.nih.gov/34559477/

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8401986/

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2649889/

Truth about dilution for urine test- ( certo , niacin , detox drinks )

 Whats the truth about Dilution for drug test ?

Do things like certo , Niacin or expensive "Detox Drinks" actually "Detox " you ? 

Methods using products like Certo, or detox drinks are essentially just adding them to a standard dilution agent like water. In the past, before synthetic urine and the internet, people used to rely on goldenseal tea for flushing. Therefore, there is no need to rely on gimmicky drinks that cost $40 and tell you to drink a gallon of water. It's important to recognize that it's the water that is diluting your urine after consuming pectin Certo, niacin, or detox drinks. Similarly, detox drinks instruct you to drink them along with multiple bottles of water, urinate a few times while continuing to drink more water before the test.

Some might wonder about the color of the urine, but in a urine test, the color is not a significant factor, and most people tend to hydrate prior to the test so they can produce urine and leave quickly. Concerning the possibility of over-hydrating and receiving a negative result due to low levels of creatinine and specific gravity (urine density), the drink itself doesn't add anything to counteract that. If you're diluting your urine by hydrating excessively, everything, including drug metabolites, color and creatinine will be diluted.

What actually happens is that you dilute your urine with water, and approximately an hour after you stop drinking excessive amounts of water, your body regulates itself and expels the excess water through urine, returning your levels to their true concentrations. This process doesn't "detox" your body as commonly claimed by those selling drinks or promoting online methods. In simple terms, the reason some people pass a urine test using these drinks and methods is often due to luck.

Alternatively, they might not have had drugs in their system to begin with, which is common for one-time users or occasional THC users who become paranoid and believe the drug will stay in their system indefinitely. However, a single use of THC typically clears from the body within three days, just like most other drugs. Many people who claim to have "passed" using these products are often one-time THC users who panicked without knowing the drug's elimination time frame. It's advisable to use a simple, inexpensive self-test urine stick before resorting to panic buying these drinks.

A laboratory drug test consists of two parts. The first part is the Validity Screen, which checks for levels of creatinine (not creatine) and specific gravity, among other factors that indicate potential sample masking or tampering. If you flush and dilute your urine but the creatinine and specific gravity levels are not significantly altered, then the urine is not considered diluted. Furthermore, if your metabolite levels are below the testing threshold, you will receive a negative result and pass the test.

However, if the specific gravity (urine density) and creatinine levels are too low, it indicates that you have consumed substances to alter your urine, which raises suspicion. Fortunately, they won't fail you outright, and you will have the opportunity to try the test again, although it may appear suspicious.

To summarize, if dilution is your only option, all you need is a $5.00 box of detox tea from any store and some inexpensive self-test sticks. Avoid self-testing when you are excessively hydrated as it may produce false negative results, as urine density validation is not performed. Remember, even a faint line on the test is considered negative for THC.

What works every single time is substituting your urine with synthetic urine. The only concern is ensuring that the synthetic urine is at the correct temperature when you submit it, but this can be easily managed with a proper container. Synthetic urine has been available for a long time, and it is a reliable method. Some may perceive it as riskier, but that is not accurate. Just practice carrying it to ensure you

What does work every single time is Synthetic urine substitution .  Your only concern is getting it in the cup at temp which is easy peezy with a proper container. Synthetic urine has been around a long time and its easy to beat. Most think it riskier but that is false.  Just practice carrying it so you know how to keep it at temp and your golden . 

One Shot Synthetic Urine Kit

Home Urine test and what the Faint Line means

Negative or Positive Test Result ?

 

ALL THC LINES ARE FAINT

 IF YOU CAN SEE IT THEN ITS NEGATIVE 

 Is your self test Qualitative or  Semi quantitative  ?

A very common question with people who are worried about seeing a faint line on the home reagent dip strip/cup  test is what does this mean ? 

These are strips which serve different purposes, such as a qualitative strip that only determines if the sample is positive or negative, such as most cheap single store bought dip strip test ( can be cup type as well).  

 

These are a basic preliminary qualitative (quality )  test and thus are not SEMI quantitative  (quantity ) test so they will not show a estimate of a quantitative result in which a color reaction is proportional to the concentration of the substance in sample carried out by comparing the test pad to a chart or react to a lighter / darker line like a CARD or CUP that is SEMI quantitative . 

SEMI quantitative self urine test

Each one of these has a different cut off level and in this case lines get lighter due to lower cut off . As you see the lowest is very faint but just as negative as the highest dark line typical of coke and opiate .

LONG STORY SHORT PEOPLE 

AS LONG AS YOU SEE TWO LINES ITS NEGATIVE  NO MATTER HOW FAINT

 

FAINT LINE ON PRELIMINARY QUALITATIVE   DOES NOT MEAN BORDERLINE

 

 If a strip also used in cups has multi colored indicators its a SEMI quantitative  (quantity ) typical of a Validation panel in addition to the drug screen . These will show If the creatinine and specific gravity (urine density),Ph ,nitates are in range ( this shows if sample is diluted) , as well as other factors to determine if sample is in human range and even shows no additive are present.

  

 

 

 

 

 

 

SVT   SPECIMEN VALIDITY TEST

Sometimes a lab will use a reagent stick validation before "accepting" the sample to sent to the lab for drug screening . regardless all samples received at lab will perform validity screen.

 a validation test verifies its ph, color, specific gravity ( verifies dilution ) creatnine level ( not creatine) and looks for nitrates or non urine AGENTS

READING THE STICK   OF SELF TEST

When reading the drug test result, you will find a line at the very top of every testing window and that is the control region (C). The test is not considered valid without this line. Result is considered negative when a colored line appears in both the control (C) and test (T) region. If the colored line appears in the control region but not in the test region, the test is considered to be positive. Nevertheless, presence of any faint line in the test region (T) will indicate negative results no matter how light it is.

Presumptive Positive: One colored line is visible in the control (C) zone for that drug. No colored line whatsoever appears in the test (T) zone, indicating a presumptive positive result for the corresponding drug.
Negative: Colored lines are visible in both the control (C) and test (T) zones, indicating that concentration of the drug is below the detection limit of the test or not present. 

 

 VAST MAJORITY OF SELF TEST ARE CHEAP SINGLE STICK REAGENT  

As long as your not diluted  and not using first morning pee you get best reading . If doing multiple during a detox do them same time of day  no heavy or excessive  fluid intake. If you see a faint line then it will most likely be a little darker with a little extra fluid  but your good just don't super dilute as this will render a DILUTE sample for re test .