Monday, September 13, 2021

Specimen validity testing the test before the (drug) test

Specimen validity testing the test before the (drug) test

Specimen validity testing (SVT) is performed on a urine drug screen specimen to detect substitution, adulteration, or dilution.

Specimen validity testing (SVT) is a critical component of urine drug screening programs. The process of collecting and testing urine samples for drugs of abuse can be susceptible to adulteration or substitution, making it challenging to ensure accurate test results. 

Specimen validity testing (SVT) is using substitution techniques,diluting urine, or adding a substance to a sample.

CharacteristicsNormal Range
Creatinine, mg/dL20–400
Specific gravity1.002–1.030
pH4.5–8.0
Temperature, within 4 minutes of voiding, °F90–100


 SVT is a multifaceted process that includes various methods and techniques.

 Dilution When someone drinks a significant amount of water shortly before providing a urine sample for donation or due to certain physiological conditions, it can lead to diluted urine. This dilution can cause drug or metabolite concentrations to fall below the initial test cutoff, potentially resulting in a false negative outcome. Dilution will mainly effect creatinine and specific gravity. 

 

Creatinine: Creatinine is a natural substance found in urine. It is created when muscle tissue breaks down and is eliminated from the body through the kidneys. Typically, the concentration of creatinine in urine falls within the range of 20 to 400 mg/dL. However, levels outside of this range can indicate factors such as excessive fluid intake, renal failure, diet, or various other medical conditions.

Specific Gravity: Specific gravity (SG) is a measure of the density of a liquid compared to the density of water. It helps determine the concentration of dissolved particles in a urine sample. A decrease in SG values may be attributed to factors like excessive fluid intake,

 

 

 Adulteration —Adding a substance to a specimen after it has been collected. The product added is designed to mask the presence of, or chemically destroy, the drug or drug metabolite that the specimen may contain. An adulterant product may be added with the intention of adversely affecting the testing reagents.

Adulterated Specimen

Urine samples with a pH level below 4.0 or above 9.0 are considered adulterated. An adulterated specimen refers to a urine sample that contains a substance not typically found in urine or an endogenous substance present at an abnormal concentration compared to normal physiological levels. 

Adulterants are products added to the urine with the purpose of masking or chemically destroying the presence of drugs or drug metabolites that the specimen may contain. Adulterant products can also be added to interfere with the testing reagents, or in the case of liquid synthetic urine, act as a preservative to maintain its stability over time.

  Some common oxidants used to attempt to alter urine drug test results are bleach, biocides, nitrate, chromate, iodate, and peroxidase. A positive result for oxidant activity should be considered when interpreting drug results.

 The most popular liquid urine product contains biocides and peroxides and if use din a reputable lab will result in “not consistent with human urine”

pH


The pH level of a urine sample indicates its acidity or alkalinity. Typically, urine pH values range from 4.5 to 9.0. However, if urine specimens are stored under unfavorable conditions such as high temperatures, the pH may rise as high as 9.5. Abnormally high or low pH levels can suggest tampering or adulteration of the sample.

When assessing urine samples, creatinine, specific gravity (SG), and pH are taken into account to determine their validity. All three criteria must fall within the range of normal human urine for a sample to be considered valid. 

 

 These tests are used to assess the validity of the urine specimen by determining its composition and consistency. A urine sample that falls outside the expected ranges for any of these parameters may be indicative of tampering or adulteration.

Urine StatesDescription
DilutedUrine creatinine ≥ 2 mg/dL but < 20 mg/dL
Specific gravity > 1.001 but < 1.030

SubstitutedUrine creatinine < 2 mg/dL
Specific gravity < 1.001 or > 1.020

AdulteratedpH < 3 or > 11
Nitrite concentration > 500 mcg/mL
Chromium concentration > 50 mcg/mL
Presence of: Halogen (bleach, iodine, fluoride), glutaraldehyde, pyridine, surfactant

In addition to these methods, SVT also involves chain of custody procedures, including documentation and tracking of the sample from collection to testing. This ensures that the sample has not been tampered with or substituted during the collection process.

The use of SVT is essential for maintaining the integrity of urine drug screening programs. It provides a reliable mechanism for detecting attempts to alter or adulterate urine specimens and ensures accurate test results. SVT can help prevent false negatives or false positives, which can have significant consequences for individuals undergoing drug testing.

It is important to note that SVT is not foolproof and can be susceptible to manipulation by individuals attempting to cheat the system. However, the use of multiple SVT methods and stringent chain of custody procedures can help minimize the risk of tampering or substitution.

In conclusion, SVT is a critical component of urine drug screening programs. It involves various methods and techniques to assess the validity of urine specimens and detect attempts to tamper with or adulterate them. The use of SVT helps maintain the integrity of drug testing programs and ensures accurate test results.



 Watch a Sample Validly Test  on  Synthetic urine  

 showing proper validity levels of the FAKE IT synthetic urine 



Sunday, October 7, 2018

Home Urine test and what the Faint Line means


Negative or Positive Test Result ?

 


ALL THC LINES ARE FAINT

 IF YOU CAN SEE IT THEN ITS NEGATIVE 


 Is your self test Qualitative or  Semi quantitative  ?

A very common question with people who are worried about seeing a faint line on the home reagent dip strip/cup  test is what does this mean ? 
These are strips which serve different purposes, such as a qualitative strip that only determines if the sample is positive or negative, such as most cheap single store bought dip strip test ( can be cup type as well).  
 
These are a basic preliminary qualitative (quality )  test and thus are not SEMI quantitative  (quantity ) test so they will not show a estimate of a quantitative result in which a color reaction is proportional to the concentration of the substance in sample carried out by comparing the test pad to a chart or react to a lighter / darker line like a CARD or CUP that is SEMI quantitative . 

SEMI quantitative self urine test

Each one of these has a different cut off level and in this case lines get lighter due to lower cut off . A
s you see the lowest is very faint but just as negative as the highest dark line typical of coke and opiate .


LONG STORY SHORT PEOPLE 

AS LONG AS YOU SEE TWO LINES ITS NEGATIVE  NO MATTER HOW FAINT

 

FAINT LINE ON PRELIMINARY QUALITATIVE   DOES NOT MEAN BORDERLINE

 

 If a strip also used in cups has multi colored indicators its a SEMI quantitative  (quantity ) typical of a Validation panel in addition to the drug screen . These will show If the creatinine and specific gravity (urine density),Ph ,nitates are in range ( this shows if sample is diluted) , as well as other factors to determine if sample is in human range and even shows no additive are present.

  



 

 

 

 

 

 

SVT   SPECIMEN VALIDITY TEST

Sometimes a lab will use a reagent stick validation before "accepting" the sample to sent to the lab for drug screening . regardless all samples received at lab will perform validity screen.

 a validation test verifies its ph, color, specific gravity ( verifies dilution ) creatnine level ( not creatine) and looks for nitrates or non urine AGENTS


READING THE STICK   OF SELF TEST


When reading the drug test result, you will find a line at the very top of every testing window and that is the control region (C). The test is not considered valid without this line. Result is considered negative when a colored line appears in both the control (C) and test (T) region. If the colored line appears in the control region but not in the test region, the test is considered to be positive. Nevertheless, presence of any faint line in the test region (T) will indicate negative results no matter how light it is.



Presumptive Positive: One colored line is visible in the control (C) zone for that drug. No colored line whatsoever appears in the test (T) zone, indicating a presumptive positive result for the corresponding drug.
Negative: Colored lines are visible in both the control (C) and test (T) zones, indicating that concentration of the drug is below the detection limit of the test or not present. 

 

 VAST MAJORITY OF SELF TEST ARE CHEAP SINGLE STICK REAGENT  

As long as your not diluted  and not using first morning pee you get best reading . If doing multiple during a detox do them same time of day  no heavy or excessive  fluid intake. If you see a faint line then it will most likely be a little darker with a little extra fluid  but your good just don't super dilute as this will render a DILUTE sample for re test . 

 






 

 

 

Risk associated with Flushing to pass Urine test


Are their risk to flushing to beat a urine drug test?





To be clear flushing is not proper detoxing so when a detox drink is called detox thats not whats really going on .


The flushing strategy involves putting large quantities of liquids into your body so that the  metabolites will be washed out as fast as possible. It is unlikely that you will remove 100% of drug metabolites using this method, but it can be effective in reducing the concentration of drug traces in your sample to below the threshold .

The Office of Drug Enforcement at the Department of Transportation (DOT) asked Dr Edward Cone from the Addiction Research Center (ARC) to investigate how effective the flushing method was. He demonstrated that drinking 1 gallon of water made the gravity of marijuana in the system drop to extremely low level and also that the marijuana test results would be negative.


levels can stay low enough for a negative for a couple hours after the water was drunk but they subsequently returned to positive. The results were the same wither with "cleansing products"  and regular drinking water. With any flushing rather if it's with certo or herbs or some 40 dollar drink you got at GNC or a head shop they all tell you to drink a gallon water afterwards. This is what causes it to dilute levels to pass.


If you are planning on using the flushing method, you will need to drink large volumes of liquids (water and anything else) before the test takes place.


Here's the issues and risk ....


For one flushing too far from a test can easily return to full levels so you have to load up on water pass a first piss containing pre water loading that still contains denser metabolite levels. Then you have to get the next piss submitted before it gets contaminated as levels return because your not  DETOXED but DILUTED. 

Failing from Dilution is a common result of imperfect flushing. Before a sample is drug screened it is VALIDATED . 

Urine Validation screens check  a sample to normal human levels for Creatinine , pH, Specific Gravity and looks for additives Nitrites, Glutaraldehyde , and Oxidants. So although the density of the drug metabolites are crashed so is the Specific Gravity or density (regardless of B vitamins to turn it yellow)  of sample and Creatinine  which has to show to prove its human. Allot of the information on flushing  on the web is pretty dated and was from before validation testing became a regular part of a drug test.   

This is why Passitkit.com doesn't sell the drinks and pills for this reason but produces  custom powdered Synthetic urine kits. The First and probably the only real uric acid synthetic urine . It has never failed a test in 18 years which also shows how reliable their delivery kits for substitution like Urine Trouble synthetic urine kit  and One Shot a temperature metered bottle perfect to pass a random urine test  at any time.   



SAMPLES OF PEOPLE WHO DRANK TOOOO MUCH WATER

Results came back negative diluted, have to do another test

Hey, I will link this on my other post, but basically I had a drug test for a job Monday. Saturday night I did the pectin method. So I got a 32 oz of red gatorade and pour certo in it and shook it up than drank it in 10 minutes than drank half a gallon of water in the next 30 minutes than took half of a b-complex pill right after than drank another half gallon after those first 40 minutes over the next 1:30 hours. Than I pissed a shit ton and tested negative at my at home test saturday night after 4 hours from starting the method. did the same thing sunday and passed again. Did the same thing Monday morning before my test but took the b-complex an hour after usual and my results came back negative but diluted. I’m think I should drink less water hit faster, maybe 32 oz in like 15 minutes and that’s it and than pop a whole b complex and piss a shit ton and still do it 4 hours before. My test is tomorrow at 2 pm, gonna the pectin method tonight once and tomorrow morning right before my test. Lmk what exactly I should do?