Everything you need to know about THC Self Test

 Opiate /cocaine have higher threshold, THC is lowest thus why lines are dark or light when negative

Both ELISA lab screens and home tests are based on the same fundamental principle of using an Enzyme-Linked Immunosorbent Assay (ELISA) to detect the presence of specific substances, such as drugs or antibodies, in a biological sample.

In both cases, the ELISA technique involves the use of specific antibodies that can bind to the target substance of interest. These antibodies are typically immobilized on a solid surface, such as a test strip or a microplate well. When a sample is introduced, any target substance in the sample can bind to the corresponding antibodies, forming an antigen-antibody complex.


In the context of an at-home drug test, ELISA typically involves the following steps:

Collection of urine sample: The individual being tested provides a urine sample using a provided collection cup or container.

Test kit preparation: The self or at-home drug test kit contains all the necessary components for conducting the ELISA. These kits usually include test strips or panels, reagents, and instructions.

Test strip/panel application: The individual dips the test strip or places the urine sample onto the designated area of the test panel. The test strip or panel typically contains specific antibodies that can bind to the target drugs or their metabolites.

Incubation: After applying the urine sample, the test strip or panel is left to incubate for a specified period, allowing the antibodies to interact with any drugs present in the urine.

Detection: The test strip or panel is then examined to determine the presence or absence of drug substances. In an ELISA-based test, this is often done by observing color changes or using a reader device that measures the intensity of the reaction.

Interpretation of results: The test results are interpreted based on the presence or absence of visible color changes or the numerical values obtained from the reader device. The test kit's instructions will provide guidance on how to interpret the results for each specific drug being tested.

It's important to note that at-home drug tests using the ELISA method can provide an indication of drug use but may not be as accurate as laboratory-based tests. Positive results obtained from an at-home test should ideally be confirmed by a certified laboratory using more advanced and precise techniques, such as gas chromatography-mass spectrometry (GC-MS) or liquid chromatography-mass spectrometry (LC-MS).



ELISA (Enzyme-Linked Immunosorbent Assay)  - SELF DRUG  are commonly used to detect the presence of drugs in biological samples, such as urine or saliva. These tests work by using specific antibodies that can bind to the target drugs, resulting in the appearance of colored lines. The presence or absence of these lines indicates whether a drug is present or not.

In a typical ELISA self drug test, there are two main lines that are observed: the test line and the control line. The test line is designed to capture the target drug, while the control line is used to verify the validity of the test. When interpreting the results, the appearance or absence of these lines is compared.

The test line will only appear if the drug concentration in the sample exceeds that threshold. In this case, a negative result would show a clear control line, indicating that the test worked properly, but no test line is visible because the drug concentration is below the detection threshold. This indicates that the person tested negative for that particular drug.

In an ELISA self-test for THC (the active compound in marijuana), a faint line, even if it is not as strong or complete as the control line, is still considered a negative result based on the predetermined threshold set for the test.

 

ELISA self-tests for THC typically have a specific threshold level for the concentration of THC or its metabolites in the sample. This threshold is the minimum concentration required for the test to produce a positive result. If the concentration of THC or its metabolites in the sample is below this threshold, the test is considered negative.

In some cases, a faint line may appear in the test region where the antibodies are designed to bind with THC or its metabolites. This faint line suggests the presence of a small amount of THC or its metabolites in the sample. However, if the concentration is below the defined threshold, it is still considered negative because it does not exceed the level required for a positive result.

It's important to note that the intensity of the test line can vary depending on factors such as the concentration of the substance in the sample and the sensitivity of the test. However, the interpretation of the test results should be based on the guidelines provided by the specific test kit manufacturer. The instructions will specify the criteria for determining a positive or negative result based on the appearance or absence of the test line.

A self test or at-home urine drug test, specifically using the ELISA (Enzyme-Linked Immunosorbent Assay) method, is a diagnostic tool designed to detect the presence of drugs or their metabolites in a person's urine sample. ELISA is a common laboratory technique used for various types of testing, including drug screening.

FALSE NEGATIVES -Do not over hydrate ,

This seems to be a common issue people thinking extra water helps detox THC ...it does not. what it does is dilute your bladder and if your over hydrated and do a self test it can give a diluted result thus a FALSE NEGATIVE  

Along with over hydrating their are other things that may influence a false result or both positive and negative as you reach a clean point .

An ELISA self-test drug test can give false negatives when plain water is used for a few reasons. ELISA (enzyme-linked immunosorbent assay) is a commonly used technique for detecting the presence of drugs or other substances in biological samples.


Lack of drug presence: ELISA tests work by detecting the presence of specific drug molecules in a sample. If a person has not consumed drugs recently or the drug concentration in their system is very low, it may fall below the detection threshold of the test. In such cases, the test may incorrectly indicate a negative result even though the person may have used drugs.


Dilution effect: Plain water, when used to adulterate or dilute a sample, can reduce the concentration of drugs present in the sample. If someone intentionally adds water to their urine sample to lower the drug concentration, it may fall below the detection limit of the ELISA test. Consequently, the test may produce a false negative result, suggesting the absence of drugs when they were actually present.


Test limitations: ELISA tests have certain limitations and can sometimes yield false negatives even when drugs are present in the sample. Factors such as the sensitivity and specificity of the test, variations in the drug metabolites or compounds being tested, and the potential for cross-reactivity with other substances can all contribute to false negatives.
ELISA LIMITS CREATE NEED FOR VALIDATION SCREEN

A validation screen, also known as a Sample Validity Test (SVT), is used to determine if a sample has been diluted, which can potentially result in a false negative result. Dilution of a sample refers to the act of adding a solvent or liquid to the original sample, thereby reducing the concentration of the target substance present in the sample.

When a sample is diluted, the concentration of the target substance may fall below the detection threshold of the test. As a result, the test may not be able to accurately detect the presence of the substance, leading to a false negative result, indicating the absence of the target substance when it is actually present.

To address this issue, a validation screen or SVT is performed as an additional step in the testing process. The SVT is designed to detect the presence of diluted samples by assessing specific parameters, such as the specific gravity, pH, or the presence of certain adulterants or markers that indicate sample tampering.

The validation screen works by comparing the results of the SVT with predefined cutoff values or ranges. If the SVT indicates that the sample is diluted beyond an acceptable threshold, the test result may be considered invalid or inconclusive. This suggests that the original sample may have been manipulated or tampered with, and further investigation or retesting may be necessary to obtain accurate results.

By including a validation screen in the testing process, laboratories can identify potential sample dilution, which helps ensure the reliability and accuracy of the test results. It serves as a quality control measure to detect and prevent false negative results that may arise due to sample manipulation or dilution.

 

"DETOX " Drinks do not detox THC

"Detox" drinks are often marketed as products that can help remove or detoxify substances like THC (the active compound in marijuana) from the body, particularly from urine samples. However, it's important to understand that these drinks do not actually eliminate or detoxify THC from the system.

When a person consumes a "detox" drink, it typically contains a combination of fluids, vitamins, minerals, and herbal extracts. These ingredients may have diuretic properties, meaning they increase urine production and frequency. As a result, the urine becomes more diluted.

The idea behind using a "detox" drink is that by diluting the urine, the concentration of THC or its metabolites may temporarily fall below the threshold level that is typically used in drug tests. This dilution can lead to a short-term window where the drug test may yield a negative result.

However, it's important to note that the effects of these drinks are temporary. The body will continue to metabolize THC over time, and the levels in urine will return to their previous concentration. Once the effects of the "detox" drink wear off, the concentration of THC metabolites in the urine will gradually rise again, potentially surpassing the threshold for detection in drug tests.

It is also worth mentioning that many drug testing laboratories are aware of the practice of using "detox" drinks and have implemented measures to detect dilution. They may use additional tests, such as checking the specific gravity or creatinine levels, to assess the integrity of the urine sample. If a sample is found to be excessively diluted, it may be considered invalid, leading to further testing or potential consequences.

In summary, "detox" drinks do not actually remove THC from the body or detoxify it. They temporarily dilute urine, which may provide a short-term window of negative test results. However, these effects are temporary, and the body will continue to metabolize THC naturally over time, leading to the return of THC metabolite levels to normal.

 

 

FAKE IT Synthetic Urine-The perfect solution when natural detoxification is not an option. 

FAKE IT Synthetic Urine offers a reliable and effective alternative for individuals who need to pass urine drug tests with confidence. Unlike risky dilution methods, which may lead to negative dilute results and raise suspicion, FAKE IT Synthetic Urine provides a consistent and undetectable substitute. With its carefully formulated composition, including key components found in real urine, FAKE IT Synthetic Urine offers a realistic solution that mimics the characteristics and properties of natural urine. Say goodbye to the uncertainties of dilution and embrace the peace of mind that comes with FAKE IT Synthetic Urine - the preferred choice for those seeking a reliable substitute when natural detoxification is not feasible.

Creatine used in Dilution for drug testing how its being done wrong.

Creatine used in Dilution  for drug testing how its being used wrong.  

 Dilution is ...

Dilution refers to the process of decreasing the concentration of a urine sample by consuming excessive fluids with the intention of manipulating a negative outcome on a drug test. Another approach is consuming substances like diuretics (e.g., coffee, cranberry juice) that increase urine output.However, it's important to note that urine drug tests have measures in place to detect dilution attempts. 

Laboratories typically analyze multiple parameters in the urine, including creatinine levels, specific gravity, and pH, to assess the sample's validity. Diluted urine often has low creatinine levels and a lower specific gravity compared to normal urine, indicating that it has been tampered with.

laboratories routinely analyze samples for creatinine and specific gravity levels. Creatinine is a waste product generated by muscles and serves as a measure of urine concentration. Specific gravity, on the other hand, measures the ratio of water to other substances in urine and is another indicator of concentration. Typical creatinine levels in urine range between 20 and 400 mg/dL, while normal specific gravity falls between 1.002 and 1.030. Specimens with a creatinine level above 2 mg/dL or below 20 g/dL, and a specific gravity lower than 1.001 or 1.003, are considered diluted.

If a test is determined to be a negative-dilute result, it is highly likely that an employer will request a second test regardless of the underlying reason. However, in some cases, employers may accept a doctor's note stating that an individual has a shy bladder, which requires consuming large amounts of water to produce a urine specimen, as a valid explanation for a dilute test.

The outcomes of a positive dilute test are considered equivalent to any other positive test. The presence of drugs is detected, and the consequences remain the same.

 In drug testing scenarios, dilution is generally considered an adulteration method and is not a reliable way to pass a drug test. Laboratories are aware of these techniques and have established protocols to detect diluted samples. A negative dilute result occurs when a urine specimen tests Negative  for a particular drug metabolite while also having a crashed specific gravity and creatinine level.

 If a diluted sample is identified, it may be deemed invalid, and further testing or consequences may be implemented, such as retesting or considering it a positive result.

 

Methods of Dilution

 Various items are inevitably added to a standard dilution of consuming a liter a hour of some sports drink and diuretics. most notably would be creatine, certo or pectin , and some times you hear people saying to use charcoal. People cant help but think they are improving things regardless if they have any proof to the claim .

adding random things are pointless for one , your still diluting with tons of fluids.

If items like pectin were so important then why don't they drink it with a cup of water instead of a gallon ?  well besides that fact it would turn to a glob in their stomach .  Pectin is just fruit fiber and it may help you create a bowel movement its not releasing THC from body fat. Same goes for charcoal, which is consumed by mouth into the stomach where it is usually used to aid in poisoning JUST CONSUMED AND STILL IN THE STOMACH.  


consuming creatine will not give you extra creatinine levels

How Creatine Works

 Creatine is a substance found naturally in muscle cells. It helps your muscles produce energy during heavy lifting or high intensity exercise.

 Taking creatine as a supplement is very popular among athletes and bodybuilders. They use it to gain muscle, enhance strength, and improve exercise performance

About half of your body’s creatine stores come from the food you eat — especially red meat and seafood — and the rest is made in your liver and kidneys from amino acids. About 95% of the body’s creatine is stored in the muscles, mainly in the form of phosphocreatine. The other 5% is found in the brain and testes .

Creatine monohydrate absorption is close to 100% [27], and when 2 g of Cr is consumed in an aqueous solution, it reaches peak plasma concentration in approximately 1 h. 

 
During exertion, your muscles convert the stored creatine molecules into energy, which assists in muscle contraction. After being used for energy, creatine is broken down into creatinine, which re-enters the bloodstream en route to the kidneys. 

 
 When you supplement, you increase your stores of phosphocreatine. This is a form of stored energy in the cells. It helps your body produce more of a high energy molecule called ATP.

Most of the creatine in the body is stored as phosphocreatine (66%), while the remaining portion exists as free creatine (33%). On average, the total creatine pool (phosphocreatine + free creatine) in the muscles of a normal-sized person is around 120 grams. Each day, about 1 to 2% (2-3 grams) of the total creatine pool is broken down and converted into creatinine within the muscles. The creatinine is then excreted in the urine.

The body has the capacity to store up to 160 grams of creatine. Therefore, one can think of the normal creatine content in the muscles (approximately 120 grams) as a gas tank that is about 75% full. Scientific studies consistently report that dietary supplementation of creatine can increase muscle creatine and phosphocreatine content by 10-40%. Consequently, supplementing with creatine allows individuals to fill up their creatine storage tank to about 150-160 grams (25-30%). However, people may respond differently to creatine supplementation, with some experiencing increases in muscle creatine content as little as 5-15%, while others may see an increase of 15-40%.

TYPES OF CREATINE 

Creatine monohydrate and creatine ester are two forms of creatine, a popular supplement known for its potential to enhance athletic performance. Let's delve into the differences in absorption and their potential impact on creatinine levels: 

1. Creatine Monohydrate: 

• Absorption: Creatine monohydrate is well-researched and has been shown to be efficiently absorbed by the body. It is the most studied and commonly used form of creatine. 

• Creatinine Levels: Creatinine is a byproduct of creatine metabolism. While creatine monohydrate supplementation may lead to a slight increase in creatinine levels, this is generally considered normal and not indicative of kidney dysfunction. It's crucial to stay hydrated when using creatine to support kidney function. 

2. Creatine Ester: 

• Absorption: Creatine ester is a form of creatine that has been chemically altered to potentially improve absorption. However, research on creatine ester is limited compared to creatine monohydrate, and some studies suggest that the ester form may not be as effective in increasing muscle creatine levels. 

 it has been found that under normal physiological conditions, creatine ethyl ester is very unstable and is largely converted into creatinine in the gastrointestinal tract, where it is also absorbed. Spillane et al¹³ demonstrate this in an experiment in which serum creatine and creatinine measurements were taken from healthy volunteers who either consumed creatine ethyl ester, creatine monohydrate or a placebo. It was found that serum creatinine levels approximately tripled after the ingestion of creatine ethyl ester, while serum creatine only marginally increased. The ingestion of creatine monohydrate caused serum creatine to increase significantly and only caused a marginal increase in serum creatinine.

Therefore, regardless of the small amount consumed during a dilution method, it does not convert into creatinine but rather gets stored. Additionally, the amount of creatinine in so-called detox drinks like Q Carbo is extremely insignificant. Your body only excretes what it breaks down, which means you need to regularly consume creatine and engage in heavy weight lifting to create the waste material that is creatinine.

 HOWEVER  HERE IS THE GOOD NEWS 

There IS a way you can get  creatinine  not creatine in volume you need during dilution. You will need to prepare it ahead of time. 20 grams of creatinine mono hydrate in orange juice, (this is needed for the acid ) and store at room temp for 3 days. Now you have creatinine that can be rejected and pissed out .  

The effect of creatine ingestion on urinary creatinine concentration: Does supplementation mask a heavy dilution?

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4170516/

https://pubmed.ncbi.nlm.nih.gov/34559477/

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8401986/

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2649889/

What are the risk with "Dilution" for Passing Urine Drug Test

What is a Dilution  method for urine drug test?

A urine drug test is a common way for employees, healthcare providers, and law enforcement agencies to detect the presence of drugs in an individuals system. One method used to try and pass a urine drug test is through dilution.  In regards to drug test, " Dilution " means  - the method to pass a urine drug screen by ingesting  large amounts of liquids to Dilute your bladder and thus dilute your metabolite levels below the cut off point. 

 

How Dilution methods work  

  The liquid need only be water but best if it is also a diuretic. Diuretics help the body get rid of excess fluid, mainly water and sodium.When diuretics stimulate kidneys to flush away sodium the body also flushes away water. This can be done by using herbal items such as dandelion root, horsetail, celery seed, green or black teas or coffee.

 By drinking large amounts of fluids, individuals aim to lower the concentration of drug metabolites in their urine sample, making it more difficult to detect. 

 What makes Dilution Risky ? 

The truth about dilution for drug testing is that it does not detoxify the body but only dilutes the urine with water. Substances like certo,  ( which require large amounts of  WATER)  and expensive detox drinks do not "detoxify" the body either are they diuretics. After consuming these the body's urine is diluted with water for a relatively short window. This creates a clear urine sample which is actually not unnatural as most drink extra prior to test but  promoters of the dilution method will say to just take B vitamins for color and creatine supplements.  However your still diluting it and if does add any color it will be a suspicious fake neon yellow.  Also Color is not a qualifying parameter so why mess with it ?  As for creatine supplements - this is another sweet spot factor in timing of consumption to get it the creatine to convert to creatinine as shown in study  "  The effects of creatine ingestion on urinary creatine concentration: Does supplementation mask heavy dilution? ". Proper use of creatine for the  purpose of suplementing during dilution requires 20 grams to be hydrated in acid or orange juice for 3 days then consumed prior to testing  so that all the cratine is converted to creatinine . " Detox" rinks like q carbo are mostly herbal tea and contain VERY little insignificant amount of creatine considering your still DILUTING IT. 

 

The Trick is that you have to ingest enough fluids to dilute your urine and metabolite levels to below threshold but NOT to point that your creatinine and Specific gravity (urine density), which is what results in a DILUTED result. Dilution results are also effected by sex and weight as seen in this study "Creatinine excretion in consecutive urine samples after controlled ingestion of water" .  Dilution is not permanent, as you urinate you remove water and body returns to previous levels. You have to hit the sweet spot during a short window, without coming up diluted.  

Urine drug screens are performed by measuring amounts of drug metabolites and if they fall over a threshold your deemed POSITIVE and if under your negative.  However Lab drug tests have two parts, including the Specimen validity testing (SVT). SVT is done to check for substitution or a sample that is not characteristic of human urine.

 The Validation screen which is performed prior to or simultaneously with drug screen. Specimen validity testing (SVT) is performed on a urine drug screen specimen to detect substances, adulteration, or dilution ( result from creatinine and specific gravity levels being crashed or under limits).

  The validation screen will also look at non human items such as sanitizing agents used in liquid urine or masking agents (adulteration). Adulteration involves adding chemicals to destroy the drug metabolite. 

 WHEN SELF TESTING  THEY DO NOT LOOK AT SPECIFIC GRAVITY AND COULD GIVE A FALSE NEGATIVE   YOU WANT TO TEST SG TO VERIFY IF OUR TOO DILUTED SO YOU KNOW YOUR WINDOW BASED ON FLUID INTAKE OVER TIME

 Alternatives to Dilution methods.

 Detoxing 

 

All drugs will be gone from urine in 3-5 days except repeated use of THC as it builds up and stores in body fat and liver.  However if you are a repeat user of THC and if your non active heavy body mass person you may not have the 1-2 month needed.

 

Adulteration 

Not even going to bother as this is a major part of the SVT 

 

 

Synthetic Urine Substitution  

This is the most effective method for passing a urine drug test every single time providing your not on observed probation  or (depending on the labs validation screen parameters ) a liquid urine as they all contain a sanitizing agent to keep them shelf stable.  Powdered synthetic urine avoid this risk but requires pre mixing which is not a major issue. I have personally done 2 pre employment and 5 randoms using synthetic and i can say once you do it and get over the jitters you feel bullet proof from then on.

 

 

"But im too scared ill get caught " 

 

 The only concern with synthetic is getting the urine in the cup at temperature.  

Simply practice carrying with water and warming pads to get rid of being nervous.

To conclude

  basically dilution is a 50 50 crap shoot with success based on your use level, amount of liquid consumed and timing it before level return to measurable levels so as to not OVER DILUTE. IF your going to attempt it I suggest you practice by doing said method and  using  a self pre test with some cheap test sticks.   

Passitkit.com over 20 years as best Synthetic urine and kits

 

 

 Passing a drug test is a common concern for individuals who use substances that are prohibited in their workplace or other areas of life. While there are many ways to cheat a drug test, not all of them are foolproof or safe. Using synthetic urine is the best option as it is a reliable and discreet way to pass a drug test without risk of dilution methods. In this article, we will discuss passitkit.com, which is considered the best synthetic urine for a drug test, and how it can help you pass your drug test.

What is passitkit.com?

Passitkit.com is a website that sells synthetic urine kits designed to help individuals pass a drug test. The kit comes with a vial of synthetic urine, a temperature strip, and a heating pad. The synthetic urine is designed to mimic real urine, and the kit is designed to ensure that the synthetic urine stays at the appropriate temperature for a drug test.

Why is passitkit.com the best choice for synthetic urine to pass a drug test?

There are many reasons why FAKE IT powdered synthetic urine is the best for a drug test. Firstly, the synthetic urine is of high quality and is designed to mimic real urine. It contains all the components that are found in real urine, including urea, uric acid, and creatinine at all proper levels adn appearance such as specific gravity , ph as well as natural golden color ,odor and surface bubbles. Secondly, the kit comes with a temperature strip (same used on collection cups) and long lasting heating pad, which ensures that the synthetic urine stays at the correct temperature for a drug test. Finally, passitkit.com has a proven 20 +year track record of helping individuals pass drug tests.

 

 

How to use passitkit.com?

Using passitkit.com is easy and straightforward. First, you need to make sure that the synthetic urine is at the correct temperature. The temperature strip will indicate whether the urine is at the right temperature. Warming pads are insulating heat sources, your body actually regulates the temp. Practicing with warm water allows you to feel comfortable carrying the kits and is half the process. We have created a page on TIPS to carrying your urine at temp all day .

Is passitkit.com safe?

Using passitkit.com synthetic urine called FAKE IT . The synthetic urine is designed to mimic real urine, and it does not contain any harmful substances only what real urine should have.

Conclusion

In conclusion, passitkit.com is the best synthetic urine for a drug test as it is of high quality, comes with a temperature strip and heating pad, and has a proven track record of helping individuals pass drug tests. The Passitkit and One Shot can be a reliable and discreet way to pass a drug test.